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Image Search Results
Journal: Frontiers in Bioengineering and Biotechnology
Article Title: Impact of Fibronectin Knockout on Proliferation and Differentiation of Human Infrapatellar Fat Pad-Derived Stem Cells
doi: 10.3389/fbioe.2019.00321
Figure Lengend Snippet: Cell proliferation capacity in human IPFSCs after FN1-KO. FN1-KO cells were compared with copGFP in cell increase (A) , percentage of cells in the S and G 2 phases (B) , and surface markers [SSEA4 (C) , CD73 (D) , CD90 (E) , CD105 (F) , and CD146 (G) ] by flow cytometry; stemness genes ( NANOG, SOX2, KLF4, BMI1, MYC, NOV, POU5F1 , and NES ) (H) , senescent genes ( CDKN1A, CDKN2A , and TP53 ) (I) , and the mesenchymal condensation gene ( CDH2 ) (J) by qPCR. GAPDH was used as an endogenous control. Data are shown as bar charts. * indicates a significant difference compared to the corresponding copGFP group ( P < 0.05).
Article Snippet: The following primary antibodies were used: CD73-APC (cat no. 17-0739-42; eBioScience, Fisher Scientific, Waltham, MA),
Techniques: Flow Cytometry, Control
Journal: Frontiers in Bioengineering and Biotechnology
Article Title: Impact of Fibronectin Knockout on Proliferation and Differentiation of Human Infrapatellar Fat Pad-Derived Stem Cells
doi: 10.3389/fbioe.2019.00321
Figure Lengend Snippet: Proliferation capacity of human IPFSCs after expansion on the dECMs deposited by FN1-KO cells. Passage 15 human IPFSCs were compared after expansion on dECMs deposited by Cas9-sgFN1a/b transduced cells (sgFN1a ECM and sgFN1b ECM, respectively) with those deposited by copGFP (copGFP ECM) and those grown on TCP (TCP) as controls in cell increase (A) , percentage of cells in the S and G 2 phases (B) , and surface markers [SSEA4 (C) , CD73 (D) , CD90 (E) , and CD105 (F) ] by flow cytometry; stemness genes ( NANOG, SOX2, KLF4, BMI1, MYC, NOV, POU5F1 , and NES ) (G) , senescence genes ( CDKN1A, CDKN2A , and TP53 ) (H) , and the mesenchymal condensation gene ( CDH2 ) (I) by qPCR. GAPDH was used as an endogenous control. Data are shown as bar charts. *indicates a significant difference compared to the corresponding copGFP group ( P < 0.05).
Article Snippet: The following primary antibodies were used: CD73-APC (cat no. 17-0739-42; eBioScience, Fisher Scientific, Waltham, MA),
Techniques: Flow Cytometry, Control
Journal: EMBO Reports
Article Title: A combinatorial panel for flow cytometry‐based isolation of enteric nervous system cells from human intestine
doi: 10.15252/embr.202255789
Figure Lengend Snippet: FACS settings and reagents.
Article Snippet: CD90 , Human ,
Techniques: Sequencing